Cell proliferation is the process whereby cells reproduce themselves by growing and then dividing into two equal copies. Growth factors employ a range of growth factor signalling pathways to activate cells to enter the cell cycle. In most cases, proliferation is mediated by growth factors operating within a highly localized environment so that only those cells in the immediate vicinity are instructed to grow. To understand how growth factors control cell proliferation, we have to consider both the nature of the signalling mechanisms and how they impinge upon the cell cycle machinery that regulates cell growth and cell division. One of the most active areas of cell signalling concerns this growth factor signalling/cell cycle interface.
An analysis of the information flow during proliferative signalling reveals that there is a complex interplay between different signalling mechanisms operating throughout the early phase of the cell cycle. In order to activate cell proliferation, growth factors have to do two things. They have to induce cells to enter the cell cycle and they also function in cell growth control by increasing cell mass through an increase in macromolecular biosynthesis. The integrity of the genome is maintained by a process of checkpoint signalling that operates to arrest the cell cycle in response to DNA damage.
Proliferation is most evident during development, when the single-celled zygote begins many rounds of growth and division to make up the cell mass of the developing organism. As the adult organism is formed, many cells stop growing when they differentiate to perform their specialized functions. In the case of neurons and muscle, this growth arrest is permanent, but, for many other cells, this arrest is temporary in that they retain the option of growing again to replace cells that are lost either through normal processes of wear and tear or through damage. There are many examples of such proliferation of specific cell types.
Interest in the signalling pathways that regulate cell proliferation increased when it became apparent that these were altered in many forms of cancer. These signalling pathways also control tumour angiogenesis. Very often, key components of the signalling pathways are proto-oncogenes that become constitutively active to function as oncogenes. Many of the tumour suppressors function as negative regulators of growth factor-mediated signalling pathways. Changes in these proliferative control mechanisms are also relevant to other diseased states, such as atherosclerosis, psoriasis, hypertension and polycystic kidney disease.
Cells enter the cell cycle through two quite distinct processes (Module 8: Figure cellular life history). One is the unique process of fertilization, and the other is cell proliferation activated by growth factors. Here the primary focus will be on the cell cycle events that occur when G0 cells are stimulated to proliferate by growth factors. There is a complex cell cycle network of signalling pathways that interact with each other to control whether or not cells will grow and divide. There is an extensive cell cycle toolkit that contains both the signalling molecules and the large number of targets that are engaged as the cell passes through the cell cycle. Growth factors act early in G1 through a growth factor signalling/cell cycle interface to engage the cell cycle signalling system that then takes over and presides over the orderly sequence of events that culminates in cell division to give two daughter cells.
Defects in the cell cycle have been linked to various diseases, such as microcephaly.
Cell cycle events
The sequence of events that occur when a cell is stimulated to grow and divide constitutes the cell cycle. Resting cells, which have zero growth (G0), have to be stimulated by growth factors in order to enter the cell cycle, which begins with the first period of growth (G1) during which it prepares for a period of DNA synthesis (S) (Module 9: Figure cell cycle). Towards the end of G1, there is a restriction point (R), which marks the point where the cell becomes irreversibly committed to traverse the rest of the cell cycle. In effect, the control of subsequent events is handed over from the growth factor-mediated signalling pathways to the internal cell cycle signalling system. The latter then controls events during the S phase and the final events of G2 and mitosis, when the cell divides. Once division is complete, control swings back to the growth factor-mediated signalling pathways. To understand how proliferation is controlled it is therefore necessary to follow the sequence of events during G1 that leads up to R. Once R is breached, cells become independent of growth factors in that further progression through the cell cycle is now in the hands of the cell cycle control system (blue arrows in Module 9: Figure cell cycle).
Once it has duplicated its chromosomes, the cell enters a second period of growth (G2) when it prepares to divide into two daughter cells during the period of mitosis (M). This M phase is separated into a series of discrete steps that begin with prophase and then pass through prometaphase, metaphase, anaphase and finally the process of cytokinesis, which divides the cell into two equal halves (Module 9: Figure mitotic events).
During prophase, the paired centrosomes begin to separate. The paired chromosomes that were replicated during the S phase begin to condense to form the sister chromatids. Multisubunit condensing complexes and histone phosphorylation are used to condense chromatin to form the two sister chromatids that are held together by cohesin molecules located at the centromeres. The nuclear membrane begins to break down. This initiation of mitosis is driven by the cyclin B/cyclin-dependent kinase 1 (CDK1) complex working in conjunction with the Polo-like kinases (Plks) (Module 9: Figure mitotic entry).
In prometaphase, the nuclear membrane disappears and the spindle begins to assemble. There are three types of spindle microtubules: the astral microtubules, the polar microtubules, which overlap each other in the middle to push the poles apart, and the kinetochore spindles that attach to the kinetochore (yellow dots) on the centromeres of the sister chromatids (Module 9: Figure mitotic events).
In metaphase, the chromatids align in one plane at the metaphase plate. The sister chromatids are attached via the kinetochore microtubule to opposite poles of the spindle. Considerable tension develops between the two chromatids because their microtubules are trying to pull them apart. Separation is prevented by the spindle checkpoint that delays separation until all the chromatids are in place.
When the spindle checkpoint control is satisfied that all the chromatids are properly aligned, a specific signal triggers the separation process that is driven by the anaphase-promoting complex (APC) that cleaves the cohesin molecules that hold the chromatids together. There are indications that anaphase is triggered by a pulse of Ca2+ (Module 9: Figure chromosome separation).
As the chromosomes separate and move towards the spindle poles, the cell begins the final process of cell cleavage: telophase. Cell division is driven by a process of cytokinesis, which depends upon a contractile ring of actomyosin that gradually contracts to form a constriction that cleaves the cell into two daughter cells (Module 9: Figure cytokinesis).
Module 9: Table cell cycle toolkit Molecular components of the cell cycle signalling system.
|Cell cycle component||Comments|
|Cyclin A1||Associates with CDK2|
|Cyclin A2||Associates with CDK2|
|Cyclin B1||Associates with CDK1|
|Cyclin B2||Associates with CDK1|
|Cyclin D1||Widely expressed, interacts with CDK4 and CDK6|
|Cyclin E1||Associates with CDK2|
|Cyclin E2||Associates with CDK2|
|Cyclin H||Associates with CDK7 to form the cyclin-dependent kinase (CDK)-activating kinase (CAK)|
|Mat1||Stabilizes the cyclin H/CDK7 complex that forms the cyclin-dependent kinase (CDK)-activating kinase (CAK) (Module 9: Figure cyclin D/CDK action)|
|Cyclin-dependent kinases (CDKs)|
|CDK7||A component of the cyclin-dependent kinase (CDK)-activating kinase (CAK) (Module 9: Figure cyclin D/CDK action)|
|p21||Expression strongly increased by p53 (Module 4: Figure Myc as a gene repressor)|
|p16INK4a||This CDK4 inhibitor shares the same gene locus as the tumour suppressor alternative reading frame (ARF)|
|p15INK4b||Expression increased by transforming growth factor β (TGF-β)|
|E2F family of transcription factors|
|E2F1||E2 promoter-binding factor 1|
|Polo-like kinases (Plks)|
|Plk1||Plays a major role in regulating mitosis (Module 9: Figure mitotic entry)|
|Wee1||Phosphorylates Tyr-15 on CDK1 (Module 9: Figure mitotic entry)|
|Myt1||Phosphorylates Thr-14 and Tyr-15 on CDK1 (Module 9: Figure mitotic entry)|
|Nek2||A kinase that may plays a priming role in phosphorylating Plk substrates|
|Citron kinase||Phosphorylates myosin light chain (MLC) during cytokinesis (Module 9: Figure cytokinesis)|
|ATM||Ataxia telangiectasia mutated|
|ATR||ATM- and Rad3-related|
|Chk1||Checkpoint kinase 1|
|Chk2||Checkpoint kinase 2|
|Aurora B||Phosphorylates MgRac-GAP and MYPT1 during cytokinesis (Module 9: Figure cytokinesis)|
|Cdc25A||Dephosphorylates CDK2 in late G1 (Module 9: Figure cell cycle signalling mechanisms)|
|Cdc25B||Dephosphorylates CDK1 at G2/M (Module 9: Figure mitotic entry)|
|Cdc25C||Dephosphorylates CDK1 at G2/M (Module 9: Figure mitotic entry)|
|Cdc14a||Dephosphorylates components of the spindle midzone complex during cytokinesis (Module 9: Figure cytokinesis)|
|Centrosome and spindle components|
|Katanin||A microtubule-severing protein|
|TCTP||A microtubule-stabilizing protein|
|Stathmin||A microtubule-destabilizing protein|
|ASPM||Abnormal spindle protein-like, microcephaly-associated|
|Cohesin||A protein that “glues” chromatids together during spindle assembly|
|Securin||A scaffolding protein that inactivates separase (Module 9: Figure chromosome separation)|
|Separase||A cysteine protease that cleaves cohesin (Module 9: Figure chromosome separation)|
|Spindle midzone complex|
|MKLP1||Mitotic kinesin-like protein 1|
|MKLP2||Mitotic kinesin-like protein 2|
|Plk1||Translocates MKLP2 to the spindle|
|ECT2||A microtubule-associated protein (MAP)|
|PRC1||Protein regulator of cytokines 1|
|KIF1||A kinesin-4 family member that interacts with PRC1|
|INCENP||Inner centromere protein|
|Survivin||See Module 9: Figure cytokinesis|
Cell cycle toolkit
The main components of the cell cycle machinery are a family of proteins known as cyclins and their associated cyclin-dependent kinases (CDKs) (Module 9: Table cell cycle toolkit). Various combinations of these two components regulate the orderly progression through the cell cycle. In addition, there are a number of CDK inhibitors (Cip/Kip and p16 families) and a number of other molecules that either regulate or carry out the downstream events, such as the pocket proteins (Rb, p107, p130), the E2F family of transcription factors and the mitotic components such as the Polo-like kinases (Plks), checkpoint kinases, regulatory phosphatases, centrosome and spindle components, chromosome separation proteins and the spindle midzone complex. The challenge is to understand how all these components come together to control the orderly sequence of events that constitutes the cell cycle. Some of these cell cycle control elements operate early in G1 and represent the targets for the proliferative signalling pathways, which are part of the cell cycle network.
Cyclin-dependent kinases (CDKs)
The cyclin-dependent kinases (CDKs) are responsible for regulating the cyclins. Each cyclin binds to a specific CDK (Module 9: Table cell cycle toolkit). The activity of the CDKs depends upon this association with the cyclins to form the cyclin–CDK complexes that control cell cycle progression (Module 9: Figure cell cycle signalling mechanisms). In addition to activating the CDKs, some of the cyclins, such as cyclin B, also determine the cellular location of the cyclin–CDK complex.
Since the cyclins lack enzymatic activity, their action is mediated by binding to cyclin-dependent kinases (CDKs) to form cyclin–CDK complexes. The cyclin–CDK complexes have multiple functions in cell cycle signalling:
Cell cycle network
There is a complex cell cycle network that has a number of signalling systems that control whether or not a cell enters the cell cycle. These signalling systems determine the choices that are available when cells exit from the cell cycle (/1Module 9: Figure cell cycle network). They can follow four possible pathways, resulting in markedly different cell fates:
It is this last option of cell proliferation that is controlled by the proliferative signalling pathways that induce resting (G0) or stem cells to enter the cell cycle. These proliferation signalling pathways are highly dynamic systems, with both positive and negative elements. Many of the positive elements are proto-oncogenes, which often are mutated to become constitutively active to form the oncogenes found in many tumour cells. Conversely, there are negative elements, and many of these are tumour suppressors that are inactivated in many tumour cells. There also are anti-proliferative signalling pathways that inhibit cells from entering the cell cycle. An important example is the Smad signalling pathway controlled by the transforming growth factor-β (TGF-β) superfamily, which not only prevents cells from entering the cell cycle, but also drives cells to differentiate.
Presiding over the operation of the cell cycle is the p53 surveillance system, which is constantly checking on the performance of all the cell cycle processes, and particularly those concerned with DNA synthesis. The p53 system is also responsive to cell stress from external sources such as irradiation, which often results in causing damage to DNA (Module 4: Figure p53 function). All such cell defects are relayed through p53, which then takes appropriate action, depending on their severity. If the damage is not too severe, p53-induced cell cycle arrestoccurs while the DNA damage is repaired (Module 9: Figure cell cycle network). However, if damage is more severe, the cell is driven towards senescence or p53-induced apoptosis.
With regard to cell proliferation, the main question to focus on is how the information flowing into the cell from the proliferative signalling pathways activates the endogenous cell cycle signalling system responsible for guiding the cell through the rest of the cell cycle. This growth factor signalling/cell cycle interface is of central importance because it deals with how the growth factor signalling systems instruct the cell cycle signalling system to proceed with another cycle of cell division.
Such a holistic view of the cell cycle network is critical for understanding the causes of cancer, because tumorigenesis develops from multiple alterations in components distributed throughout the signalling network that regulates the cell cycle (Module 12: Figure cell cycle network and cancer).
Growth factor signalling/cell cycle interface
The interface between the growth factor signalling pathways and the cell cycle signalling system is a critical event in the control of cell proliferation. It is during this process that information flowing in from the cell surface sets in motion the sequence of events that culminates in cell division. The cell cycle network illustrates how the cell cycle is controlled by a number of signalling systems (Module 9: Figure cell cycle network). An overview of how the proliferative signalling pathways engage the cell cycle signalling pathway is shown in Module 9: Figure G1 proliferative signalling. A key event is the activation of cyclin D control of G1 progression, which then activates the E2F transcriptional system, which is critical for initiating the events that lead to DNA synthesis. The nature of the molecular interface between these signalling systems and the cell cycle signalling system is shown in more detail in Module 9: Figure proliferation signalling network.
The cyclins are the primary targets of the signals coming into the cell from growth factor receptors on the cell surface. Proliferative signalling pathways drive cells into the cell cycle by activating events early in G1. One of these pathways depends on Ras and the mitogen-activated protein kinase (MAPK) signalling pathway, and another is the Wnt signalling pathway that acts through β-catenin to increase the level of the transcription factor Myc. The latter then increases the transcription of cyclin D, which is an early component of the cell cycle signalling machinery (Module 9: Figure cell cycle dynamics). The cyclin D then acts on retinoblastoma protein (Rb) to remove its inhibitory effect on E2F, which then activates the formation of cyclin E and cyclin A, which are responsible for driving the cell into the cell cycle (Module 9: Figure proliferation signalling network). Myc also increases the expression of the inhibitor of DNA binding (Id) proteins that play a key role in the proliferation–differentiation switch (Module 8: Figure proliferation–differentiation switch).
In contrast with the positive effects that certain growth factors have in initiating the cell cycle, other growth factors such as transforming growth factor-β (TGF-β) act by inhibiting the cell cycle signalling pathway by interfering with cyclin activation. The transforming growth factor-β inhibition of cell proliferation depends upon the up-regulation of the cyclin-dependent kinase (CDK) inhibitor p15 that inhibits cyclin E and cyclin A. The Smads also act to prevent the formation of the transcription factor Myc, which is a key regulator of cell proliferation (Module 4: Figure Myc as a gene activator).
The p53 surveillance system, which has such an important role in checking that the cell cycle proceeds normally with no errors, also exerts its inhibitory effects on the cell cycle by interfering with the cyclins to induce either G1 or G2/M arrest (Module 9: Figure proliferation signalling network). p53 produces p21, growth-arrest and DNA-damage-inducible protein 45 (GADD45) and the 14-3-3 protein. The p21 inhibits cyclin E to bring about G1 arrest, whereas 14-3-3 protein and GADD45 are responsible for G2/M arrest. The 14-3-3 protein binds to Cdc25, and thus prevents it from activating cyclin B, whereas GADD45 also inactivates cyclin B to prevent it from initiating the onset of mitosis (Module 9: Figure cell cycle signalling mechanisms). In addition, p53 promotes the transcription of apoptotic factors that initiate apoptosis.
The PtdIns 3-kinase signalling pathway contributes to the build-up of cyclin D by increasing the stability and the expression of the cyclin D mRNA. Another important function of this PtdIns 3-kinase pathway is to control some of the key regulators of the cell cycle. For example, the downstream effector of this pathway, protein kinase B (PKB), phosphorylates the forkhead box O (FOXO) transcription factor to reduce its ability to activate transcription of the CDK inhibitor p27. This leads to a reduction in the level of p27, which remains low throughout the rest of the cell cycle (Module 9: Figure cell cycle dynamics), and thus facilitates the operation of the cell cycle signalling events.
The downstream elements of the proliferative and anti-proliferative signalling pathways thus interface with the cell cycle by controlling the activities of the cyclin/CDK complexes, which are central components of the cell cycle signalling system.
Cell cycle signalling
Cell cycle signalling is responsible for controlling the orderly sequence of events that occur when a cell is stimulated to grow and divide. Central features of the signalling system are the waves of cyclin expression that occur during the course of the cell cycle (Module 9: Figure cell cycle dynamics). Since the cyclins lack enzymatic activity, their action is mediated by binding to cyclin-dependent kinases (CDKs) to form cyclin/CDK complexes. Each cyclin binds to a specific CDK (Module 9: Table cell cycle toolkit). The activity of the CDKs depends upon this association with the cyclins to form the cyclin/CDK complexes that control cell cycle progression (Module 9: Figure cell cycle signalling mechanisms). In addition to activating the CDKs, some of the cyclins, such as cyclin B, also determine the cellular location of the cyclin/CDK complex.
Before the CDKs can begin to function as serine/threonine kinases, they have to be activated by the cyclin-dependent kinase (CDK)-activating kinase (CAK). The different complexes can also be regulated by more specific phosphorylation/dephosphorylation reactions that are particularly important in the cyclin B/cyclin-dependent kinase 1 (CDK1) control of mitosis. In addition, the cyclin/CDK complexes can be regulated by a variety of cyclin/CDK-associated proteins. For example, Mat1 stabilizes the cyclin H/CDK7 complex, which is a component of CAK. The cyclin-dependent kinase (CDK) inhibitors are particularly important in regulating the cyclin/CDK complexes that operate early in the G1 phase. The sequential activation of different cyclin/CDK regulatory complexes is responsible for the orderly progression of the cell cycle, as illustrated in Module 9: Figure cell cycle signalling mechanisms.
Cyclin B/cyclin-dependent kinase 1 (CDK1) activation
Once S phase is complete, the cell enters the second growth phase (G2) when it prepares to begin mitosis. This entry into mitosis depends upon the activation of the cyclin B/cyclin-dependent kinase 1 (CDK1) complex, which has been kept inactive by phosphorylation of CDK1 by the Wee1 and Myt1 kinases. These inhibitory phosphates are removed by the Cdc25B/C phosphatase, and the active cyclin B/CDK1 complex enters the nucleus, where it activates nuclear envelope breakdown (NEB) and spindle assembly. It also contributes to metaphase arrest, which ensures that anaphase is prevented until all the chromatids are assembled on the spindle. When this is achieved, the final phase of chromosome separation and cytokinesis is triggered by the activation of the anaphase-activating complex (APC). An increase in Ca2+, acting through Ca2+/calmodulin-dependent protein kinase II (CaMKII), triggers this process. Once activated, the APC degrades cyclin B, thus cancelling the inhibitory effect of the cyclin B/CDK1 complex on anaphase, and it also hydrolyses securin to release the enzyme separase that is responsible for hydrolysing the cohesin that sticks the chromatids together. Once they are separated, the chromatids once again become functional chromosomes as they move away from each other towards the opposite poles. Cytokinesis is triggered by the formation of a spindle midzone complex that activates the RhoA signalling mechanisms responsible for activating contraction of actin and myosin to complete the processes of mitosis.
The sequence of events just described in Module 9: Figure cell cycle signalling mechanisms provides a signalling roadmap composed of a number of events that are described in more detail in subsequent sections. The first event is the cyclin D control of G1 progression. The level of cyclin D, which is low in the resting cell (G0), increases rapidly following growth factor stimulation. As the level of cyclin D rises, it binds to CDK4 or CDK6 and the cyclin D/CDK4/6 complex enters the nucleus, where it drives the transcription of a number of cell cycle components that control the next phases of the cycle. For example, cyclin E controls G1 progression and DNA synthesis and is followed by cyclin A control of DNA synthesis. Cyclin E/CDK2 acts together with cyclin A/CDK2 to initiate DNA synthesis. Once DNA synthesis has been switched on, most attention is focused on this process of replication at the expense of other cellular processes such as gene transcription, which are switched off. Little else happens until DNA synthesis is complete, whereupon the cell leaves the S phase and enters the G2/M phases, where cyclin B/cyclin-dependent kinase 1 (CDK1) controls mitosis. During this final M phase, there are three main events:
Cyclin D controls G1 progression
The cell cycle is driven by the waves of cyclin formation that begin when growth factors activate the transcription of cyclin D.
The way in which cyclin D controls G1 progression depends upon the transcriptional activation and expression of cell cycle signalling molecules.
Cyclin D is a key component of the growth factor signalling/cell cycle interface, because it is the main target of growth factor signalling pathways (Module 9: Figure cell cycle signalling mechanisms). Of the three cyclin D isoforms, cyclin D1 is the most widely expressed in cells. Their partners cyclin-dependent kinase 4 (CDK4) and 6 (CDK6) are constitutively expressed, but remain inactive until the level of cyclin D rises early in G1 and begins to activate transcription through the sequence of events shown in Module 9: Figure cyclin D/CDK action:
In addition to controlling the activity of the E2F transcription factors, the Rb family also regulates transcription by remodelling chromatin through its ability to pull in the histone deacetylases (HDACs). The Rb-associated protein RbAp48 contributes to the complex between Rb and HDAC. The phosphorylation of Rb results in the removal of the HDACs, which are replaced by the histone acetyltransferases (HATs) that open up chromatin so that transcription of cell cycle components can occur.
In addition to controlling the expression of cyclin E, the Rb/E2F signalling systems also control a number of other genes, such as cyclin A, and thus set up the next waves of cyclin formation to propel the cell cycle forward. One of the processes to occur is that cyclin E controls G1 progression and DNA synthesis.
As is apparent in Module 9: Figure cell cycle dynamics, the cyclin D level remains high throughout the cell cycle, which means that the daughter cells are able to immediately enter another cell cycle once they complete mitosis, assuming that growth factors are present. However, if growth factors are withdrawn during the cell cycle, the level of cyclin D will fall, and the daughter cells enter a G0 state.
Cyclin E controls G1 progression and DNA synthesis
Cyclin E is a nuclear protein that pairs up with cyclin-dependent kinase 2 (CDK2) to form the cyclin E/CDK2 complex that has two main functions (Module 9: Figure cell cycle signalling mechanisms). Firstly, it contributes to G1 progression by phosphorylating the retinoblastoma (Rb) family of proteins. This process sets up a positive-feedback loop, because two of the genes activated by Rb phosphorylation are cyclin E itself and the E2F1–E2F3 transcription factors. The cyclin-dependent kinase (CDK) inhibitors also play an important role in cyclin E/CDK2 activation. In particular, cyclin E/CDK2 can remove p27, which is one of the major inhibitors that limit its activity. It phosphorylates p27, which is then degraded by the SCFSkp2 ubiquitin ligase.
Its second action is to contribute to the onset of DNA synthesis. One of its substrates is the transcription factor nuclear protein mapped to the ataxia telangiectasia locus (NPAT), which regulates the transcription of histone that will be required during the S phase. It also phosphorylates cyclic AMP response element-binding protein (CREB)-binding protein (CBP)/p300 to enhance its histone acetyltransferase (HAT) activity, which is essential for the transcription of many of the genes that are required for driving the cell into DNA synthesis. Finally, it can initiate the process of centrosome duplication by phosphorylating nucleophosmin to cause it to leave the centrosome.
The activity of the cyclin E/CDK2 complex is regulated by Cdc25A, which is one of the dual-specificity Cdc25 phosphatases that act by removing inhibitory phosphates on CDK2.
Cyclin E is somewhat unstable, and its level in the cell is determined by the balance between synthesis and proteolysis. The latter is carried out by two separate ubiquitination pathways. Monomeric cyclin E is degraded by the pathway that depends on the Cul-3 protein. On the other hand, the cyclin E/CDK2 complex is degraded by the SCFFbw7 ubiquitin ligases. As the cell cycle proceeds, these proteolytic mechanisms gain the upper hand and the level of cyclin E falls, and its role is then taken over by the cyclin A control of DNA synthesis.
Roscovitine, which is a potent inhibitor of cyclinE/CDK2, is being tested as an anticancer drug. It may also prove useful for treating polycystic kidney disease.
Cyclin A control of DNA synthesis
Cyclin A is a nuclear protein of which there are two isoforms. The cyclin A1 is normally restricted to germ cells, whereas the cyclin A2 functions in most cells to control both the G1/S and G2/M transitions. In effect, it provides a link between the early events in G1 and the final events that occur during the G2/M phase, when cyclin B/cyclin-dependent kinase 1 (CDK1) controls mitosis.
Cyclin B/cyclin-dependent kinase 1 (CDK1) controls mitosis
The cyclin B/cyclin-dependent kinase 1 (CDK1) complex controls the final events of the cell cycle, during which the cell divides into two daughter cells (Module 9: Figure cell cycle signalling mechanisms). This mitotic phase can be divided into three main phases. The first phase is nuclear envelope breakdown and spindle assembly. The second is chromosome separation at anaphase, and, finally, there is the process of cytokinesis, during which the cell divides into the two daughter cells.
Nuclear envelope breakdown and spindle assembly
The entry into mitosis at the G2/M interface depends upon the activation of the cyclin B/cyclin-dependent kinase 1 (CDK1) complex. The main events that control passage through the G2/M boundary are illustrated in Module 9: Figure mitotic entry:
Microtubule organizing centre (MTOC)
As its name implies, the microtubule organizing centre (MTOC) functions to organize the polymerization of microtubules into networks that control various cellular processes:
Spindle-assembly checkpoint signalling
Errors in chromosome segregation can lead to aneuploidy (abnormal chromosome numbers), which is a feature of many aggressive tumours. Such errors are prevented by a dynamic surveillance mechanism known as the spindle-assembly checkpoint, which ensures that segregation does not begin until all the chromatid pairs are correctly aligned on the mitotic spindle (Module 9: Figure spindle assembly checkpoint). This checkpoint operates by controlling the activity of the anaphase-promoting complex (APC) responsible for initiating chromosome separation at anaphase segregation (Module 9: Figure chromosome separation).
The spindle-assembly checkpoint signalling pathway, which is still being worked out, has to detect the existence of free kinetochores and relay this information to the APC complex to inhibit its activity. The following hypothesis has been proposed to explain how this signalling pathway might operate. The proteins Mad2 and BubR1 appear to be the sensors that detect the free kinetochores on the incomplete spindle (Module 9: Figure spindle assembly checkpoint). These proteins are also able to bind the Cdc20 to prevent it from activating APC. Before Cdc20 can be activated, it must be released from this inhibitory action of Mad2/BubR1 and must also by polyubiquinated by a combination of APC and E2 ubiquitin ligase UbcH10. The p21Comet facilitates the binding of UbcH10 to Cdc20. Even though these two ligases are constitutively active, they are not able to ubiquitinate Cdc20 because any added ubiquitin groups are immediately removed by an ubiquitin-specific protease 44 (Usp44). There appears to be a futile cycle of ubiquitination and deubiquitination, which is biased in favour of the latter, thus ensuring that the Cdc20 is kept inactive.
As soon as the spindle is complete, the complexes located on the kinetochores dissociate to liberate the Cdc20. The latter is then rapidly ubiquitinated and this enables it to stimulate APC to initiate the process of chromosome separation at anaphase (Module 9: Figure chromosome separation).
Chromosome separation at anaphase
The spindle-assembly checkpoint, which ensures that anaphase is delayed until all the chromatids are lined up on the spindle equator (metaphase plate), is switched off once all the chromatids are assembled on the spindle (Module 9: Figure spindle assembly checkpoint). Chromosome separation can now begin through a process that appears to be triggered by an increase in Ca2+ that initiates a signalling sequence that activates the anaphase-promoting complex (APC) through the sequence of steps shown in Module 9: Figure chromosome separation:
The final process of mitosis is cytokinesis, which is responsible for cell division when the cell cleaves into two equal daughter cells. As the chromosomes move towards the poles during anaphase, the spindle remains in the form of the overlapping polar microtubules. Proteins that are concentrated at this midzone of the spindle then determine the division plane during cytokinesis. The exact process and how it is controlled is still being worked out, but it appears to depend upon four clearly defined processes that begin with assembly and activation of a spindle midzone complex, which results in the activation of RhoA that then controls assembly of the actomyosin contractile ring and the activation of contraction. Formation of the cleavage furrow depends upon the trafficking and insertion of membrane vesicles.
Assembly and activation of a spindle midzone complex
The polar microtubules that are left behind after the chromosomes move towards the poles function to assemble the spindle midzone complex that determines the division plane (Module 9: Figure cytokinesis). This midzone complex is made up of three main groups. There is the Aurora B/inner centromere protein (INCENP)/survivin/Csc1 group, the protein-regulating cytokinesis 1 (PRC1)/KIF1 group and the mitotic kinesin-like protein 1 (MKLP1)/MgcRac GTPase-activating protein (MgRacGAP) group. Some of these components, such as PRC1, MKLP1 and INCENP, are phosphorylated by the cyclin B/cyclin-dependent kinase 1 (CDK1) complex and lie inactive in the cytoplasm. In the case of MKLP1, its phosphorylation inhibits its motor activity, which thus prevents it from binding to the spindle. When the cyclin B/CDK1 complex is degraded at anaphase (Module 9: Figure chromosome separation), these phosphorylated proteins are dephosphorylated by the phosphatase Cdc14a and are then incorporated into the spindle midzone complex (Module 9: Figure cytokinesis). The site of insertion of the MKLP1/MgcRacGAP complex may depend upon the Aurora B complex. Aurora B may play a role in stimulating MgcRacGAP, which is a component of the signalling system that controls the assembly of the actomyosin contractile ring.
Assembly of the actomyosin contractile ring
The spindle midzone complex determines the position where the contractile ring is formed, and it also helps to control the activation of RhoA, which is responsible for initiating the assembly of actomyosin fibres (Module 9: Figure cytokinesis). Actin is assembled through an interaction between the proteins formin and profilin. The formin normally functions to cap the barbed ends of the actin filaments to prevent them from growing. However, the activated RhoA-GTP binds to the N-terminal region of formin, its autoinhibition is removed, and the filament begins to grow by nucleating the actin monomers carried in by profilin. As the activation of contraction proceeds and the contractile ring contracts down, there is an overabundance of actin, and this is then reduced by cofilin, which acts to destabilize and severe the redundant actin filaments.
Activation of contraction
Contraction of the contractile ring is controlled by phosphorylation of the myosin light chains (MLCs) on Ser-19 and Thr-18, which is carried out by a number of mechanisms (Module 9: Figure cytokinesis). This phosphorylation of MLC enables the head of the non-muscle myosin II motor to interact with actin to bring about contraction of the ring. One of the phosphorylation pathways is carried out by the RhoA signalling mechanism, which results in stimulation of Rho kinase (ROCK) that activates contraction by stimulating MLC or by inhibiting myosin phosphatase targeting subunit 1 (MYPT1). The latter is a targeting protein that positions the protein phosphatase 1c (PP1c) at its site of action (Module 5: Table PP1 regulatory, targeting and inhibitory subunits and proteins), which, in this case, is on the myosin molecule, where it can remove the activating phosphates on MLC. The MYPT1 is inhibited following its phosphorylation by both Rho kinase and by Aurora B. Citron kinase, which is another Rho-effector kinase, has also been implicated in the control of myosin activity.
MLC can also be phosphorylated by myosin light chain kinase (MLCK), which is stimulated by Ca2+ acting through calmodulin (CaM). There certainly are remarkable changes in the distribution of CaM during mitosis (Module 9: Figure CaM redistribution at mitosis). The high concentration at the spindle poles suggests some role in the process of anaphase. The appearance of CaM at the site of the cleavage furrow is consistent with a role for Ca2+ in controlling cytokinesis (Module 9: Figure cytokinesis).
Imaging experiments have observed localized Ca2+ signals in the vicinity of the cleavage furrow. This localized Ca2+ signal may be released from inositol 1,4,5-trisphosphate (InsP3) receptors because an extra cleavage furrow was induced by injecting microsomes rich in these receptors near the surface membrane of a Xenopus embryo (Module 9: Figure InsP3 receptors and cell cleavage).
Trafficking and insertion of membrane vesicles
The monomeric G protein Rab11, operating through the Rab11-family interacting protein 2 (FIP2), plays a role in controlling the trafficking and insertion of new vesicles to form the cleavage furrow (Module 9: Figure cytokinesis). Annexin A11 also seems to play a role in the trafficking of vesicles to the plasma membrane, and this would suggest that it is a Ca2+-mediated process.
Cyclin-dependent kinase (CDK)-activating kinase (CAK)
The cyclin-dependent kinase (CDK)-activating kinase (CAK) is a complex composed of cyclin H, CDK7 and Mat1, which is a RING finger protein that stabilizes the complex. CAK functions to activate the cyclin/CDK complexes by phosphorylating a threonine residue located on the T-loop region of the CDKs. This T-loop region inhibits the CDKs by covering up their ATP-binding sites. CAK moves this T-loop out of the way to activate the CDKs. CAK plays a critical role in activating the CDKs. For example, it activates cyclin D/CDK4/6 once it enters the nucleus (Module 9: Figure cyclin D/CDK action).
Polo-like kinases (Plks)
The Polo-like kinases (Plks) (Module 9: Table cell cycle toolkit), which are expressed from G2 onwards and peak at the time of mitosis, are serine/threonine kinases that play a critical role during the M phase by controlling many aspects of mitosis, including nuclear envelope breakdown and spindle assembly, chromosome separation at anaphase and the process of cytokinesis. They have an N-terminal kinase domain and two C-terminal Polo-box (PB) domains (PB1 and PB2) responsible for binding to their substrates. These PB domains form a phosphopeptide-binding motif. In its resting state, these PB domains bend around to interact with the kinase catalytic region to keep it inactive. Activation of the Plks depends upon the phosphorylation of Thr-210 in the T-loop of the kinase domain. They also have a D-box motif, which controls their destruction by the anaphase-promoting complex (APC). The Plks, particularly Plk1, play an important role in controlling various events during the M phase, such as entry into mitosis (Module 9: Figure mitotic entry) and the separation of the chromatids at anaphase (Module 9: Figure chromosome separation).
Cyclin-dependent kinase (CDK) inhibitors
There are two quite distinct families of cyclin-dependent kinase (CDK) inhibitors (Module 9: Table cell cycle toolkit) that play a critical role in regulating the cell cycle: the INK4 family and the Cip/Kip family.
The INK4 family functions to inhibit cyclin D/CDK4/6 complexes (Module 9: Figure cell cycle signalling mechanisms). There is considerable interest in this family because p15 functions in the transforming growth factor β (TGF-β) inhibition of cell proliferation. There also is interest in p16INK4a, which is a specific inhibitor of CDK4, because it is a tumour suppressor that plays a role in the development of some cancers. p16INK4a has also been implicated in the signalling pathways of compensatory hypertrophy.
The activity of cyclin E/CDK2 is controlled by the Cip/Kip family, which is older than the INK4 family in evolutionary terms, consists of p21, p27 (KIP1) and p57 (KIP2). The p53-induced cell cycle arrest depends upon the transcriptional activation of various cell cycle components, such as p21 (Module 4: Figure p53 function). Similarly, the hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] inhibits parathyroid gland hyperplasia by increasing the expression of p21 (Module 7: Figure PTH secretion).
Mutations of p57 have been linked to Beckwith–Wiedemann syndrome.
The p27 also plays an important role in the activation of the cyclin E/CDK2 complex (Module 9: Figure cell cycle signalling mechanisms). The onset of cell proliferation depends upon a rapid fall in the level of p27 early in G1 (Module 9: Figure cell cycle dynamics).
The pocket proteins, represented by the retinoblastoma protein (Rb) family (Module 9: Table cell cycle toolkit) contains three proteins: Rb/p105, p107 and p130/Rbl2. Rb play a central role in regulating the cell cycle through their control of the E2F family of transcription factors that regulate expression of a number of cell cycle components, such as cyclin E, cyclin A, Cdc25A and E2F itself (Module 9: Figure cell cycle signalling mechanisms). The Rb family and the E2F family bind to each other (Module 9: Figure Rb–E2F interactions) to set up a complex web of interactions that function to both activate and repress transcription of a large number of genes. The Rb family acts by regulating the activity of the E2F family, with Rb acting as an activator, whereas p107 and p130/Rbl2 are repressors.
The pocket proteins have an important role in maintaining the cell in a G0 state by repressing the E2F genes (Module 9: Figure cyclin D/CDK action). Most attention has focused on Rb, which is a nuclear protein whose activity is regulated through its phosphorylation state. During the resting G0 phase, hypophosphorylated Rb binds to E2F to repress its transcriptional activity. However, when cyclin D/cyclin-dependent kinase 4/6 (CDK4/6) begins to phosphorylate Rb, the latter leaves the promoter site to allow E2F1–E2F3 to transcribe a variety of genes that function in the cell cycle (Module 9: Figure cell cycle signalling mechanisms). In the case where Rb family members act to control repressors such as E2F4 and E2F5, the phosphorylation of p107 and p130/Rbl2 removes these repressors. Therefore, there are two main mechanisms whereby the Rb family regulates transcription. Rb allows the E2F1–E2F3 isoforms to exert their activator role, whereas p107 and p130 remove the repressor activity of E2F4 and E2F5 (Module 9: Figure cyclin D/CDK action).
E2F family of transcription factors
The family of E2F transcription factors (Module 9: Table cell cycle toolkit) plays a critical role in cell proliferation by controlling the transcription of many of the key components of the cell cycle. The E2Fs function together with their binding partners DP1 and DP2. The E2F family can be divided into those that are strong transcriptional activators (E2F1, E2F2 and E2F3a) and those that act as repressors (E2F4–E2F7) (Module 9: Figure Rb–E2F interactions). Both the activators and the repressors are controlled by the family of pocket proteins. Retinoblastoma protein (Rb) acts to regulate E2F1–E2F3, whereas the repressors E2F4 and E2F5 are regulated by the pocket proteins p107 and p130 (Module 9: Figure cyclin D/CDK action). E2F6 and E2F7 act independently of the pocket proteins.
The Rb/E2F families set up a network that not only controls cell proliferation, but also participates in checkpoint controls, differentiation and apoptosis.
DNA damage and checkpoint signalling
DNA damage occurs frequently during the life of a cell and can lead to mutations and the development of cancer. There are different types of damage that give rise to different types of DNA lesions that are then dealt with by a sophisticated set of repair mechanisms often referred to as the DNA damage response (DDR) pathway. Since these repair processes can take some time to complete, a serious problem arises in cells that are actively proliferating. It would be disastrous if cells began DNA synthesis or mitosis while repairing their DNA. Consequently there are DNA damage checkpoint signalling systems that operate to co-ordinate the processes of DNA repair with the different phases of the cell cycle.
DNA damage results in genotoxic stress caused by both exogenous and endogenous agents resulting in different types of lesion. These lesions are then recognized by components of the different repair mechanisms. This DNA damage is caused by two main groups of agents. Firstly, there are exogenous agents such as chemicals, ultraviolet (UV) and ionizing radiation (IR). Secondly, there are endogenous agents such as reactive oxygen species (ROS) that induce DNA breaks, oxidation of bases, mismatches caused by replication defects and strand breaks caused by collapse of the replication fork. Double strand breaks (DSBs), which are among the deadliest of lesions, are caused by multiple factors including replication fork collapse induced by blocking lesions caused by ROS, normal genomic rearrangements that occur during meiosis or V(D)J recombination or by the physical stress exerted on dicentric chromosomes as they are pulled apart at mitosis. When such DSBs occur during the S phase, the high levels of CDKs will induce resection of the DSBs resulting in the appearance of single-stranded DNA (ssDNA). Stalling of the DNA polymerase can also result in ssDNA tracts when the replicative helicase is uncoupled from the advancing fork. The checkpoint signalling pathways are triggered by either the DSBs or the ssDNA. The Fanconi anaemia/BRCA pathway also seems to play a central role in both DNA repair and cell cycle control.
Genotoxic stress activation of NF-κB signalling provides another mechanism for protecting cells following DNA damage.
Defects in the DDR signalling pathways have been linked to a number of disorders such as Ataxia telangiectasia (AT) syndrome where mutations in ataxia telangiectasia mutated (ATM) results in defects in the repair of double strand breaks (DSBs).
DNA damage activates a checkpoint signalling system that has components similar to those found in many other cell signalling pathways. Components of the checkpoint signalling toolkit (Module 9: Table checkpoint signalling toolkit) are organized into signalling pathways that relay information from the sites of DNA damage to various downstream effectors that can temporarily arrest the cell cycle. The DNA lesions, such as the double-strand breaks (DSBs) or single-stranded DNA (ssDNA), provide the stimuli that are recognized by ‘receptors’. The latter are then coupled through transducers/amplifiers to activate the checkpoint kinases that function as messengers that relay information to the various effectors, which are components of the cell cycle machinery. The key components of the checkpoint signalling network are the phosphoinositide 3-kinase related kinases (PIKKs) represented by Ataxia-telangiectasia mutated (ATM), ATM and RAD3-related, and DNA-dependent protein kinase. It is these three PIKKs that feature significantly in checkpoint signalling systems that will be illustrated by considering G1 checkpoint signalling to DNA double-strand breaks (DSBs) and S and G2/M checkpoint signalling to single stranded DNA.
Module 9: Table checkpoint signalling toolkit Checkpoint signalling toolkit.
|Ku 70 and Ku80||One of the sensors that recognizes DSBs (Module 9: Figure G1 checkpoint signalling)|
|RAD 50||One of the sensors that recognizes DSBs (Module 9: Figure G1 checkpoint signalling)|
|RPA||Replication protein A coats single stranded DNA (ssDNA) (Module 9: Figure S/G2 phase checkpoint signalling)|
|Transducers/amplifiers||Phosphoinositide 3-kinase related kinases (PIKKs)|
|ATM||Ataxia-telangiectasia mutated (ATM)|
|ATR||ATM and RAD3-related|
|DNA-PK||DNA-dependent protein kinase|
|MRE11||Meiotic recombination protein-11|
|Nbs1||Nijmegen breakage syndrome protein-1 responsible for recruiting ATM|
|MDC1||Mediator of DNA damage checkpoint protein-1 is an adaptor that functions in G1 checkpoint signalling (Module 9: Figure G1 checkpoint signalling)|
|ATRIP||ATR-interacting protein is an adaptor that functions in S/G2 checkpoint signalling (Module 9: Figure S/G2 phase checkpoint signalling)|
|RAD17-RFC||RAD17-replication factor C functions as a clamp loader for the 9-1-1 complex|
|RAD-9||A component of the 9-1-1 complex|
|RAD1||A component of the 9-1-1 complex|
|HUS1||A component of the 9-1-1 complex|
|Claspin||Adaptor protein that connects ATR to CHK1 (Module 9: Figure S/G2 phase checkpoint signalling)|
|Checkpoint kinases (CHKs)|
|CHK1||Checkpoint kinase 1|
|CHK2||Checkpoint kinase 2|
G1 checkpoint signalling to DNA double-strand breaks (DSBs)
DNA double-strand breaks (DSBs) are among the deadliest of lesions and are responsible for triggering the following two G1 checkpoint signalling cascades (Module 9: Figure G1 checkpoint signalling):
The protein acetylase TIP60 also plays a role in growth arrest by acetylating p53 on lysine-120.
S and G2/M checkpoint signalling to single-stranded DNA
An ATR/checkpoint 1 signalling pathway, which occurs mainly during the S and G2 phases of the cell cycle, operates during the process of homologous recombination. The primary trigger that initiates this checkpoint signalling response is the existence of single-stranded DNA (ssDNA) as outlined in the following sequence of events (Module 9: Figure S/G2 phase checkpoint signalling):
Fanconi anaemia/BRCA pathway
The Fanconi anaemia/BRCA pathway operates during DNA damage that is induced by DNA cross-linking and other forms of genotoxic stress. The components of this pathway came to light during the analysis of Fanconi anaemia, which is a genetically heterogeneous disease that results in chromosome instability. There is a Fanconi anaemia complementation group (FANC) that has at least 12 components (A, B, C, D1, D2, E, F, G, I, J, L and M). These FANC proteins, which seem to function as two separate complexes (the green and yellow groups of proteins shown in Module 9: Figure Fanconi anaemia pathway), co-operate with each other in the operation of a signalling system that responds to various defects in DNA. The pathway is particularly evident during DNA synthesis when the replication fork stalls due to defects such as a DNA cross-link. Just how all these proteins function is still being worked out and the following hypothesis illustrates some of the key features of the pathway (Module 9: Figure Fanconi anaemia pathway):
Mutations in components of the Fanconi anaemia/BRCA pathway are responsible for the genetically heterogeneous disease Fanconi anaemia.
Genotoxic stress activation of NF-κB signalling
Genotoxic stress resulting from DNA damage caused by a variety of insults, such as ionizing radiation and anticancer drugs, can induce a protective pathway based on activation of the NF-κB signalling pathway (see Step 10 in Module 2: Figure NF-κB activation). The activity of the ubiquitin E3 ligase Parkin may be particularly relevant with regard to cell survival because one of the genes activated by NF-κB is optic atrophy 1 (OPA1), which is critical for maintaining the cristae in mitochondria and thereby prevents apoptosis (Module 5: Figure OPA1 and mitochondrial cristae remodelling).
Resting cells in a zero growth phase (G0) are induced to re-enter the cell cycle by growth factors that function by stimulating a process of cell proliferation (Module 9: Figure cell cycle network).
Spatial and temporal aspects feature significantly in the activation of cell proliferation by growth factor signalling pathways (Module 9: Figure G1 proliferative signalling). The spatial problem concerns the fact that growth factors acting at the cell surface have to transfer information to internal targets, many of which are found in the nucleus. With regard to the temporal problem, the signalling pathways have to operate over long periods in G1. This question of the time it takes to stimulate cells to enter the cell cycle is also apparent during the completion of meiosis at fertilization, where Ca2+ spikes have to persist for 2 h before the zygote is committed to begin its growth and development. Another example is Ca2+ signalling in lymphocytes, where a similar long period of stimulation is required to drive cells into the cell cycle. Therefore the information flow during proliferative signalling is adapted to transmit information from growth factor receptors to the nucleus over long periods of time.
Information flow during proliferative signalling
Most of the signalling pathways used to transmit information into the cell are made up of sequential elements, some of which are sufficiently stable and diffusible to disperse information deep into the cell, thus solving the spatial problem. These early signalling events are driven by the so-called competence growth factors in that they set the stage for the action of the later progression growth factors (Module 9: Figure G1 proliferative signalling). Solution of the temporal problem has two elements. Firstly, the signalling mechanisms show little desensitization, thus enabling them to operate over prolonged periods. Secondly, the signalling pathways trigger a cascade of gene transcriptional events that serve to maintain the flow of information during the critical G1 period. One way of doing this is to induce the expression of additional signalling pathways that operate later in G1. It is during this transcriptional cascade that the progression growth factors and their receptors are put in place to provide additional signalling pathways that carry information in from the outside to drive some of the later cell cycle signalling elements responsible for initiating DNA synthesis.
In summary, growth factors use a number of signalling mechanisms to initiate the process of cell proliferation. Early in G1, these signalling pathways transmit information into the nucleus to trigger a transcriptional cascade that culminates in the phosphorylation of retinoblastoma protein (Rb) at the restriction point. As the role of the growth factor-dependent signalling pathways decline during G1, the onus for driving the cell cycle to completion is gradually handed over on to the endogenous cell cycle signalling mechanisms based on the cyclin cascade (blue arrows in Module 9: Figure cell cycle). These cell cycle events are set in motion by a number of growth factor signalling pathways.
Growth factor signalling pathways
Growth factors use a variety of signalling pathways to transmit information into the nucleus. In describing these pathways, the emphasis will be placed on the way in which information travels from the cell surface to the cytosolic and nuclear targets that co-ordinate the onset of DNA synthesis, as outlined in Steps 1–10 in Module 9: Figure growth factor signalling:
The signalling mechanisms summarized in Module 9: Figure growth factor signalling represent a proliferative signalling toolkit from which specific systems are assembled to control the proliferation of specific cell types.
K+ channels and cell proliferation
There are many examples of K+ channels playing a role in controlling cell proliferation. The primary candidate for carrying out this role is the intermediate-conductance (IK) channel KCa3.1 (Module 3: Table properties of Ca2+-sensitive K+ channels), which is one of the Ca2+-sensitive K+ channels. The primary action of KCa3.1 is to hyperpolarize the membrane and thereby maintain the driving force for the entry of external Ca2+, as has been demonstrated for lymphocytes (Module 9: Figure T cell Ca2+ signalling).
Proliferation is reduced by toxins (charybdotoxin and iberiotoxin) or inhibitors that block these K+ channels. Overexpression of these channels has been found in many tumours.
Cell growth control
The growth factors that control cell proliferation have two major actions. They must exert both cell cycle control and cell growth control. The latter is concerned with the control of the macromolecular synthetic events that are necessary for cells to double their size prior to mitosis. This control of growth is complex, because not only is it stimulated by growth factors, but also it is regulated by nutrients such as amino acids and by the energy state of the cell. What is remarkable about these different regulatory inputs is that they all impinge upon a common regulatory system based on the mammalian target of rapamycin (TOR), which then controls the target of rapamycin (TOR), which has a major role in regulating protein synthesis by phosphorylating both ribosomal S6 protein kinase 1 (S6K1) and eukaryotic initiation factor 4E (eIF4E)-binding protein (eIF4E-BP1). TOR is a protein kinase that forms two complexes: TOR complex 1 and TOR complex 2. Most information is available for TOR complex 1, which consists of TOR, regulatory-associated protein of TOR (raptor), G protein-like protein (GβL) and proline-rich PKB/Akt substrate 40 kDa (PRAS40). This TOR complex 1 is regulated through two separate signalling mechanisms (Module 9: Figure target of rapamycin signalling). One pathway is activated by hormones and growth factors and uses signalling components such as the tumour suppressor proteins tuberous sclerosis complex 1 and 2 (TSC1/2) and the Ras-homologue enriched in brain (Rheb). The other pathway is regulated by and increase in circulating amino acids that activate a Ca2+ signalling mechanism. The following sequence of events indicates how these two pathways function to activate TOR to regulate protein synthesis (Module 9: Figure target of rapamycin signalling):
Tuberous sclerosis complex 1 and 2 (TSC1/2)
The tuberous sclerosis complex 1 and 2 (TSC1/2) plays a critical role in regulating cell growth control because it co-ordinates many of the inputs that regulate the control of protein synthesis by the protein kinase target of rapamycin (TOR) (Module 9: Figure target of rapamycin signalling). TSC is a heterodimer composed of TSC1 (also known as hamartin), which contains a coiled-coil structure, and TCS2 (also known as tuberin), which is a GTPase-activating protein (GAP). TSC1 and 2 are phosphorylated by protein kinase B (PKB) during the action of growth factors.
TSC1 and TSC2 are considered to be tumour suppressor genes because they act by inhibiting the growth promoting signals from nutrients. The genes encoding TSC1/2 are inactivated in the autosomal dominant disorder tuberous sclerosis.
Target of rapamycin (TOR)
The target of rapamycin (TOR), which is also referred to as mammalian target of rapamycin (mTOR), derives its name from the fact that it is inhibited by the drug rapamycin that is a potent inhibitor of cell proliferation. Some analogues of rapamycin are being tested as anti-tumour agents. Rapamycin exerts its inhibitory action by first binding to the immunophilin FK506-binding protein (FKBP) of 12 kDa (FKBP12) (Module 9: Figure target of rapamycin signalling).
TOR is a serine/threonine protein kinase, which operates as a nutrient-sensitive cell cycle checkpoint. The highly conserved kinase domain is located at the C-terminus close to an FRB domain that binds the FKBP12. The activity of TOR is switched off, and cell proliferation ceases under conditions when amino acid levels are low or when energy is limiting. This inhibition of TOR is also responsible for the onset of autophagy(Module 11: Figure autophagy)
The N-terminal region of TOR contains a large number of HEAT repeats. These helical repeat units fold into an extended superhelical scaffold-like structure that may provide interfaces for interactions with other molecules. TOR is organized into two complexes. In the TOR complex 1, TOR is associated with a regulatory-associated protein of TOR (raptor), G protein-like protein (GβL) and a proline-rich PKB/Akt substrate 40 kDa (PRAS40). Raptor is a scaffolding protein that provides a bridge between TOR and its downstream targets of eIF4E-BP1 and ribosomal S6 protein kinase 1 (S6K1). The TOR complex 2 contains TOR together with GβL, rapamycin-insensitive companion of TOR (rictor), mammalian stress-activated protein kinase (SAPK)-interacting protein 1 (mSin1) and protein observed with rictor (protor).
Protein synthesis is often referred to as translation because it depends on a process of converting the codon sequences of mRNA being translated into an amino acid polypeptide chain. This translation process consists of three discrete mechanisms: initiation, elongation and termination.
The activity of eIF-2α is a key control point for eukaryotic protein synthesis and is altered in response to various stresses such as viral infection, nutrient deprivation and the endoplasmic reticulum (ER) stress signalling response (Module 2: Figure ER stress signalling). Many of these cellular stresses inhibit protein synthesis by activating different members of the eIF2 protein kinase family such as Haem-regulated inhibitor (HRI), double-stranded (ds) RNA activated protein kinase (PKR), general control non-derepressible-2 (GCN2) and protein kinase RNA–like endoplasmic reticulum kinase (PERK). In the case of PERK, which is a dual-specificity protein kinase, misfolded proteins within the lumen of the ER induce dimerization during which there is a tyrosine autophosphorylation reaction (Module 9: Figure regulation of eIF-2α cycling). Once it is phosphorylated, PERK can then phosphorylate eIF-2α that then binds to eIF2B and prevents it from carrying out its role of exchanging GDP for GTP and thus effectively represses translation.
PERK is inactivated by protein tyrosine phosphatase 1B (PTP1B), which is located on the surface of the endoplasmic reticulum. An interesting aspect of this dephosphorylation reaction is that it can be inhibited by a sulfhydration of the hyper-sensitive cysteine-215 by the gastrotransmitter hydrogen sulfide (H2S).
Eukaryotic initiation factor 2 (eIF-2)
The eukaryotic initiation factor 2 (eIF-2), which consists of three subunits (eIF-2α, eIF-2β and eIF-2γ), has a critical role in protein synthesis in that it facilitates attachment of mRNA to the ribosome (Module 9: Figure regulation of eIF-2α cycling). The activity of eIF-2 is inhibited by phosphorylation of serine-51 on the α-subunit and this markedly reduces protein synthesis. Four kinases have been identified that are capable of reducing protein synthesis by phosphorylating eIF-2α. While all four kinases share a conserved kinase domain, they have divergent regulatory regions that enable them to respond to different types of cell stress (Module 9: Figure regulation of eIF-2α cycling):
Proliferation of specific cell types
Cell proliferation is evident in many different cell types. Stem cells are specialized for cell proliferation. For example, in the intestine, there are nests of undifferentiated stem cells that are constantly proliferating to replace absorptive cells that are sloughed off from the ends of the villi. In the immune system, lymphocyte activation plays a vital role during clonal expansion of unique lymphocytes programmed to fight off specific infections. There also are many differentiated primary cells that perform their specific functions while retaining the option of returning to the cell cycle (Module 8: Figure cellular life history). In all of these examples, it is clear that cell proliferation is carefully orchestrated to maintain cellular homoeostasis.
The following are some examples of in vivo cell proliferation events that can be induced with the appropriate stimuli:
The immune system employs a highly sophisticated chemical surveillance system to recognize and combat foreign substances. The foot soldiers of this defence force are the lymphocytes, which are separated into thymus-derived cells (T cells) and bone marrow-derived cells (B cells). The T cells act against intracellular pathogens (cell-mediated immunity), whereas the B cells deal with extracellular pathogens (humoral immunity). There are two main types of T-cell: the cytotoxic cells, which can kill infected cells, and the helper cells, which, as their name implies, help B cells to fight infections by providing specific cytokines. From a signalling point of view, T cell activation and B cell activation are of interest because they are examples of how cells are stimulated to proliferate in response to a specific stimulus, in this case antigen. In addition, these cells can also respond to antigen either by dying through apoptosis or by entering an unresponsive state known as anergy. Exactly which course they take depends on the nature of the signalling information they receive through a variety of receptor pathways.
T cell activation
T cell activation depends upon a sequential series of events that begin when the circulating T cell responds to the cytokines emanating from the source of an infection. Through a process of T cell chemotaxis, the T cell homes in on the infected area, where it makes intimate contact with the antigen-presenting cell to form an immunological synapse. The chemotactic response and the formation of the synapse are very dependent upon T cell cytoskeletal reorganization. As the two cells come into contact, the major histocompatibility complex II (MHCII) molecules on the antigen-presenting cell present the antigenic peptides to the T cell receptors to initiate the T cell receptor (TCR) signalling responsible for T cell activation. Activation through the TCR is facilitated by the CD28 co-stimulatory pathway.
T cell chemotaxis
Circulating T cells detect and move towards sites of infection by a process of chemotaxis. This chemotactic response is usually driven by a gradient of chemokines (Module 9: Figure T cell chemotaxis). Resting T cells have a round shape with numerous small microvilli. This uniform shape, which is maintained by a cortical web of microfilaments, undergoes a rapid change when the T cell begins to detect chemokines. This T cell cytoskeletal reorganization induces a clearly defined polarity that contributes to the process of chemotaxis.
T cell cytoskeletal reorganization
Remodelling of the actin cytoskeleton plays a key role in T cell activation. It is responsible for the dramatic changes in cell shape that occur during T cell chemotaxis, and it comes into play at a later stage to help assemble the immunological synapse. These cytoskeletal rearrangements are controlled by an interplay between the PtdIns 3-kinase signalling pathway and the PtdIns4,5P2 signalling cassette. The reorganization of the actin cytoskeleton that occurs during T cell chemotaxis is very similar to that seen in neutrophil chemotaxis.
As chemotaxis proceeds and the two cells begin to make contact with each other, there is another cytoskeletal reorganization that forms the immunological synapse (Module 9: Figure T cell actin scaffold).
When the T cell makes contact with potential antigen-presenting cells, it carries out an initial surveillance that causes a change in shape (see Sequence a–d in Module 9: Figure T cell shape change). If this target is a naïve cell, there is no further response, and the T cell moves away. However, if the T cell recognizes an antigen-presenting cell, there is a dramatic change in shape as it throws out pseudopodia, almost totally engulfing its target (see Sequence e–h in Module 9: Figure T cell shape change). This rapid change in shape is accompanied by a large Ca2+ transient, which is thought to stabilize the interaction with the antigen-presenting cell.
The antigenic peptides responsible for activating the T cell are not free in solution, but are held by the major histocompatibility complex II (MHCII) molecules embedded on the surface of the antigen-presenting cell (Module 9: Figure T cell chemotaxis). As a result of the process of chemotaxis, the two cells come together to form an immunological synapse made up of highly organized adhesive and signalling components (Module 9: Figure immunological synapse structure). These adhesive components, such as lymphocyte function-associated antigen (LFA-1), form a protective outer ring surrounding an inner core containing the signalling components. Within this synapse, therefore, molecules appear to be segregated into ‘supramolecular activation clusters’ (SMACs). The peripheral SMAC (pSMAC) contains the adhesion molecules, whereas the central SMAC (cSMAC) contains the signalling components.
An important aspect of the immunological synapse is the polymerization of actin to provide an ‘actin-scaffold signalling highway’ that helps in the relay of information from the cSMAC into the cell. The organization of the actin cytoskeleton depends upon the recruitment of the Wiskott–Aldrich syndrome protein (WASP) and the WASP-interacting protein (WIP) into the immunological synapse. This translocation may be assisted by a Crk-like (CrkL) adaptor protein. The ability of WIP to inhibit the activity of WASP may be alleviated by phosphorylation of the former by protein kinase Cθ (PKCθ), which is known to play a role in T cell activation. This actin scaffold may also act to reorientate the microtubule organizing centre (MTOC) so that it faces towards the antigen-presenting cell. This actin remodelling was also found to be necessary for the activation of Oria1, which is essential for lymphocyte activation (Module 3: Figure STIM-induced Ca2+ entry).
The important principle is that the efficient functioning of the signalling system depends upon the construction of an elaborate scaffold made up of both the cytoskeleton and a range of scaffolding proteins that contributes to the molecular organization of a functional T cell receptor (TCR) (Module 9: Figure TCR signalling). One of the important signalling events regulated by the cytoskeleton is Ca2+ entry, which seems to be controlled by actin remodelling carried out by a signalling pathway that includes Vav, Rac and WASP verprolin homologous 2 (WAVE2) (Module 3: Figure STIM-induced Ca2+ entry).
There is a disruption in lymphocyte signalling in Wiskott–Aldrich syndrome, which results from mutations of WASP that result in a disruption in the formation of the immunological synapse.
T cell receptor (TCR) signalling
The T cell receptor (TCR) signalling processes responsible for the activation of proliferation contain a bewildering array of components. The main components of the T cell signalling toolkit are collected together into functional groups (Module 9: Table T cell signalling toolkit). Many of these components are collated together on a spatiotemporal map, which provides a visual framework to illustrate how they interact with each other to drive T cells into DNA synthesis (Module 9: Figure T cell signalling map). In effect, this is a spatiotemporal flow diagram that begins at the top left with the arrival of the antigen and ends with DNA synthesis in the bottom right-hand corner. Using such a map, it is possible to determine approximately where and when different signalling components function during the activation process.
The signalling events proceed through a conventional sequence, as shown in the green panel on the left of the map. The activation process begins with the ligands [e.g. antigen held on the major histocompatibility complex II (MHCII)] that bind to the large TCR complex that is coupled to transducers to create the messengers that relay information through various signalling cassettes to activate the cytosolic and nuclear effectors that initiate the transcriptional cascade that finally results in DNA synthesis. Some of the main components of T cell signalling are outlined below.
T cell ligands
T cells respond to a number of ligands, most of which are held on the surface of the antigen-presenting cells (Module 9: Figure T cell signalling map). The primary ligand for T cell activation is the antigen itself. The antigen is taken up by the antigen-presenting cell, where it is chopped up into short fragments (8–15 amino acids) that are bound to the major histocompatibility complex II (MHCII). The MHCII molecules then display this antigen fragment on the cell surface, where it is recognized by the T cell receptor (TCR). In effect, this peptide fragment acts as a growth factor to initiate cell proliferation. For a successful response, however, the T cell must be stimulated by additional ligands, such as CD86, that act through the CD28 receptor.
T cell receptors, precursors and transducers
The T cell receptor (TCR) is a complicated multimolecular complex. It begins with a few fixed components, but as soon as it binds antigen, it draws in a large number of additional transducing and scaffolding components to build a huge macromolecular complex, which has also been referred to as a ‘transducisome’, which functions to relay information into the cell (Module 9: Figure TCR signalling). The TCR consists of transmembrane proteins (α, β and ζ) that are associated with the CD3 components (CD3γ, δ and ε). This TCR complex is associated with the accessory co-receptor CD4 that binds to the major histocompatibility complex II (MHCII). The TCRα and TCRβ subunits form an immunoglobulin-like receptor that is responsible for recognizing the antigen fragment held in the jaws of the MHC. Activation of the TCR creates a large macromolecular complex responsible for relaying information through a number of signalling cassettes.
Since the α and β subunits of the TCR have very short cytoplasmic tails, they are incapable of engaging the signal transducing elements, and this function is carried out by other members of the receptor. The CD3 subunits (γ, δ and ε) and the ζ subunits have long cytoplasmic chains that contain immunoreceptor tyrosine-based activation motifs (ITAMs) (red bars), which provide the docking sites to assemble the receptor scaffold. The ‘glue’ that directs and holds the scaffold together is provided by the phosphate groups that are added sequentially to the various ITAMs. The assembly of this signalling scaffold follows an orderly sequence of events:
T cell receptor (TCR) signalling cassettes
The T cell receptor (TCR) activates cell proliferation by relaying information to the nucleus using many of the conventional growth factor signalling pathways (Module 9: Figure T cell signalling map):
T cell receptor (TCR) Ca2+ signalling
One of the major signals for activating lymphocyte proliferation is Ca2+, which is generated by the inositol 1,4,5-trisphosphate (InsP3)/Ca2+ signalling cassette. The phosphate group of LAT binds to the Src homology 2 (SH2) domain of phospholipase Cγ1 (PLCγ1) through the conventional mechanism for activating the γ isoform of PLC (Module 2: Figure PLC structure and function). The PLCγ1 is thus positioned close to the membrane, where it can begin to initiate the following sequence of events (Module 9: Figure T cell Ca2+ signalling):
An important point to stress is that the Ca2+ signalling pathway has to remain active for at least 2 h in order to stimulate lymphocytes to proliferate. During this activation period, the Ca2+ signals are presented as prolonged oscillations (Module 9: Figure T cell Ca2+ oscillations). An increase in Ca2+ is thus a key regulator of cell proliferation. Lymphocyte activation and smooth muscle cell proliferation provide paradigms for such a role for Ca2+ in controlling cell proliferation.
T cell activation provides a good example of the link between K+ channels and cell proliferation. At least two K+ channels are responsible for hyperpolarizing the membrane to maintain the electrical gradient necessary for the prolonged influx of external Ca2+ through the Oria1 channels (Module 9: Figure T cell Ca2+ signalling). There is KV1.3 and the intermediate-conductance (IK) channel (KCa3.1). Hypoxia results in a decrease in the expression of the KV1.3 channel and this can lead to a decrease in cell proliferation.
The Ras/mitogen-activated protein kinase (MAPK) cassette
(Module 9: Figure T cell signalling map). The mitogen-activated protein kinase (MAPK) signalling pathway plays an important role in lymphocyte activation. The growth-factor-receptor-bound protein 2 (Grb2) subunit is drawn into the complex where, together with Son-of-sevenless (SoS), it helps to assemble the membrane complex that initiates the extracellular-signal-regulated kinase (ERK) signalling pathway (Module 2: Figure ERK signalling).
Small GTP-binding proteins and the cytoskeleton
(Module 9: Figure T cell signalling map) The scaffold protein Src homology 2 domain-containing leukocyte protein of 76 kDa (SLP-76) binds Rac, Vav and Nck, which function to organize the cytoskeleton. For example, Vav is a guanine-nucleotide exchange factor (GEF) for the Rho family of monomeric G proteins such as Rac, which function by organizing the cytoskeleton (Module 2: Figure Rac signalling). Modification of the cytoskeleton may also depend on the ability of Vav to bind talin and vinculin, which function to anchor the cytoskeleton to the plasma membrane. In addition, Vav may influence Ca2+ signalling by acting through Rho to stimulate the PtdIns4P 5-kinase (PtdIns4P 5-K) to provide the precursor lipid PtdIns4,5P2 used by the PLCγ1 and PtdIns 3-kinase signalling cassettes (Module 9: Figure TCR signalling).
T cell PtdIns 3-kinase signalling cassette
(Module 9: Figure T cell signalling map). The regulatory subunit of the PtdIns 3-kinase has Src homology 3 (SH3) and two Src homology 2 (SH2) domains that enable it to bind to the activated receptor, thus enabling its associated catalytic subunit to begin to phosphorylate PtdIns4,5P2 to the second messenger PtdIns3,4,5P3 (PIP3) (Module 2: Figure PtdIns 3-kinase signalling). One of the functions of PIP3 is to facilitate the activation of phospholipase Cγ1 (PLCγ1) by binding directly to the enzyme, and also by activating the protein inducible T cell kinase (Itk) (Module 9: Figure TCR signalling).
CD28 co-stimulatory pathway
Activation of the T cell receptor (TCR) by itself is not sufficient to induce cell proliferation, even though it is capable of sending information down a number of prominent signalling cassettes. To complete its role in initiating events early in G1, the TCR complex requires assistance from the co-stimulatory pathway (Module 9: Figure T cell signalling map). In addition to facilitating the proliferative signal, the CD28 pathway also prevents the TCR from entering into an unresponsive state that has been referred to as clonal anergy. Just how the CD28 pathway acts to prevent the TCR from becoming unresponsive is still very much a mystery.
CD28 is a transmembrane glycoprotein that functions as a homodimer linked together through disulphide bonds in the extracellular domain. The ligands for CD28 are CD80 and CD86, which are members of an immunoglobulin family located in the membrane of the antigen-presenting cell (Module 9: Figure TCR signalling). The cytoplasmic domain of CD28 lacks enzymatic activity, but it does contain tyrosine residues that become phosphorylated following stimulation, and which may then recruit signalling elements such as PtdIns 3-kinase. There also is an indication that CD28 may activate the sphingomyelin signalling pathway, resulting in the activation of a number of pathways including the JNK pathway.
The inducible costimulatory molecule (ICOS), which is also known as CD278, is related to CD28 and also acts by providing positive signals during T cell activation. CD28 and ICOS have unique and overlapping functions and act synergistically to promote cell activation. CD28 is expressed in resting T cells, whereas the expression of ICOS is induced once T cells are activated. For example, ICOS is induced in follicular helper T cells (TFH cells) during B cell differentiation in the lymph node (Module 8: Figure B cell maturation signalling). ICOS is activated by the ICOS ligand (ICOSL), also known as B7RP-1, which acts by stimulating the PtdIns 3-kinase signalling pathway to induce the production of effector cytokines such as interleukin 4 (IL-4) and interleukin 21 (IL-21).
Module 9 Table T cell signalling toolkit An inventory of the major signalling components used to stimulate T cell proliferation.
|MHC class II||Major histocompatibility complex II|
|CD86||Transmembrane glycoproteins (on antigen-presenting cells) responsible for stimulating CD28|
|CD80||Transmembrane glycoproteins (on antigen-presenting cells) responsible for stimulating CTLA4|
|TCRα||T cell receptor α subunit|
|TCRβ||T cell receptor β subunit|
|CD3γ||The γ chain of the CD3 component of the TCR|
|CD3δ||The δ chain of the CD3 component of the TCR|
|CD3ε||The ε chain of the CD3 component of the TCR|
|TCRζ||The ζ chain of the TCR|
|TCRη||The η chain of the TCR|
|CD4||An accessory co-receptor that binds to MHC class II|
|CD8||An accessory co-receptor that binds to MHC class I|
|CD28||A co-stimulatory receptor that responds to CD86|
|ICOS||Inducible co-stimulatory molecule that acts synergistically with CD28|
|IL-2Rα||IL-2 receptor α subunit|
|IL-2Rβ||IL-2 receptor β subunit|
|IL-2Rγ||IL-2 receptor γ subunit|
|PITP||Phosphatidylinositol transfer protein|
|PI 4-K||Phosphatidylinositol 4-kinase|
|PIP 5-K||Phosphatidylinositol 4-phosphate 5-kinase|
|Lck||A non-receptor tyrosine kinase (a member of the Src family)|
|Fyn||A non-receptor tyrosine kinase (a member of the Src family)|
|Itk||Inducible T cell kinase, T cell-specific tyrosine kinase|
|ZAP70||ζ-Associated protein of 70 kDa, a tyrosine kinase closely related to ZAP70|
|Syk||Spleen tyrosine kinase closely related to ZAP70|
|CD45||A transmembrane tyrosine protein phosphatase|
|CSK||C-terminal Src kinase|
|SHP||Src homology 2 (SH2) domain-containing protein tyrosine phosphatase|
|G protein components|
|Rac||A monomeric G protein|
|Cdc42||A monomeric G protein|
|Vav||A guanine nucleotide exchange factor (GEF) for Rho family GTPases|
|LAT||Linker for activation of T cells|
|SLP-76||Src homology 2 (SH2)-domain containing leukocyte protein of 76 kDa|
|GADS||Grb2-related adaptor protein|
|CrkL||Crk-like scaffolding protein|
|Nck||A scaffolding protein|
|CARMA1||A scaffolding protein that links PKCθ to the activation of the NF-κB signalling pathway (Module 9: Figure TCR signalling)|
|Bcl10||A scaffolding protein that links CARMA1 to the activation of the NF-κB signalling pathway|
|MALT1||Mucosa-associated lymphoid tissue protein-1 that functions with CARMA1 and Bcl10 to the activation of the NF-κB signalling pathway (Module 9: Figure TCR signalling)|
|InsP3R1||Inositol 1,4,5-trisphosphate receptor type I|
|InsP3R2||Inositol 1,4,5-trisphosphate receptor type II|
|InsP3R3||Inositol 1,4,5-trisphosphate receptor type III|
|IP3 3-K||InsP3 3-kinase|
|IP3 5-Pase||InsP3 5-phosphatase|
|KCa3.1||Ca2+-activated intermediate-conductance (IK) K+ channel|
|SERCA||Sarco/endo-plasmic reticulum Ca2+-ATPase|
|PMCA||Plasma membrane Ca2+-ATPase|
|STIM1||Stromal interaction molecule 1 (Module 3: Figure SOC signalling components)|
|Orai1||The main component of the Ca2+ release-activated Ca2+ (CRAC) channel (Module 3: Figure SOC signalling components)|
|DAG kinase||Diacylglycerol kinase|
|PKCα||Protein kinase Cα|
|PKCε||Protein kinase Cε|
|PKCζ||Protein kinase Cζ|
|PKCθ||Protein kinase Cθ|
|PI 3-kinase pathway|
|PI 3-K||Phosphatidylinositol 3-kinase|
|PTEN||Phosphatase and tensin homologue deleted on chromosome 10|
|SHIP||Src homology 2 (SH2) domain-containing inositol polyphosphate 3-phosphatase|
|PKB||Protein kinase B (also known as Akt)|
|FRAP||FK506-binding protein 12 (FKBP12) rapamycin-associated protein|
|p70S6K||70 kDa ribosomal S6 kinase|
|p85S6K||85 kDa ribosomal S6 kinase|
|Ras||A small GTP-binding protein|
|Shc||Src homology 2 (SH2)-domain-containing protein (an adaptor protein for Ras activation)|
|Grb2||Growth factor receptor-bound protein 2|
|Raf-1||Cellular homologue of the v-Raf oncogene (a serine/threonine protein kinase)|
|MEK||MAPK/extracellular-signal-regulated kinase (ERK) kinase|
|MAPK||Mitogen-activated protein kinase|
|JAK1||Janus kinase 1|
|JAK3||Janus kinase 3|
|STAT3||Signal transducer and activator of transcription 3|
|STAT5||Signal transducer and activator of transcription 5|
|Ceramide||A second messenger released from sphingomyelin|
|CAPK||Ceramide-activating protein kinase|
|CAPP||Ceramide-activated protein phosphatase|
|WASP||Wiskott–Aldrich syndrome protein|
|WAVE2||WASP verprolin homologous; related to WASP and regulates actin cytoskeleton|
|Arp2/3||Actin-related proteins 2 and 3|
|CaMKIV||Ca2+/calmodulin-dependent protein kinase IV|
|Proteasome||A multisubunit complex responsible for degrading ubiquitin/rotein conjugates|
|NFAT||Nuclear factor of activated T cells (cytoplasmic component)|
|IκB||Inhibitor of NF-κB|
|E2F||E2 promotor binding factor|
|Jun||A nuclear transcription factor (a component of activating protein 1)|
|Fos||A nuclear transcription factor (a component of activating protein 1)|
|p21||A 21 kDa inhibitor of cyclin-dependent kinases|
|p27kip1||A 27 kDa inhibitor of cyclin-dependent kinases|
|Cyclin E||A cell cycle protein that functions late in G1 to control onset of DNA synthesis|
|CDK2||Cyclin-dependent kinase 2 (a threonine/serine protein kinase)|
|Rb||Retinoblastoma gene product|
The purpose of this T cell signalling toolkit, which is by no means complete, is to highlight the fact that cell proliferation depends upon the co-operation of a large number of signalling components. The description of the abbreviations often helps to describe their different functions. The way in which many of these components contribute to T cell activation to transfer information from the cell surface into the cytoplasm and nucleus is summarized in Module 9: Figure T cell signalling map.
The purpose of this T cell signalling toolkit, which is by no means complete, is to highlight the fact that cell proliferation depends upon the co-operation of a large number of signalling components. The description of the abbreviations often helps to describe their different functions. The way in which many of these components contribute to T cell activation to transfer information from the cell surface into the cytoplasm and nucleus is summarized in Module 9: Figure T cell signalling map.
Signalling lymphocyte activation molecule (SLAM)
There are nine genes in the signalling lymphocyte activation molecule (SLAM) family (SLAM1–9) that play an important role in controlling the various interactions that lymphocytes make with different cells. They belong to an immunoglobulin superfamily that is located on the cell surface of haematopoietic cells. Most of the family are type I transmembrane glycoproteins whereas SLAMF2 is attached to the membrane through a glycosylphosphatidylinositol anchor and functions as a receptor for SLAM4. All of the other SLAMs interact in a homophilic mechanism in that their ectodomains can interact with each other. The cytoplasmic tails of six of these receptors (SLAM1, SLAM3–7) carry unusual intracellular tyrosine-based switch motifs (ITSMs). When the SLAMs interact with each other, these ITSMs are phosphorylated and recruit various SH2-domain-containing adaptor proteins such as the SLAM-associated protein (SAP) and EWS/FLI activated transcript-2 (EAT-2).
The SLAM/SAP signalling pathway functions in a large number of lymphocyte interactions to control processes such as the development of T cell lineages, natural killer T cells (NKT cells) and the affinity maturation of B cells. For example, during B cell differentiation in the lymph node, there is an increase in the expression of SAP and SLAM in the TFH cells that are responsible for the B cell selection within the germinal centre (Module 8: Figure B cell maturation signalling).
SLAM-associated protein (SAP)
SLAM-associated protein (SAP) and the related EWS/FLI activated transcript-2 (EAT-2), which is expressed mainly in antigen-presenting cells, are SH2-domain adaptor proteins that bind to the phosphotyrosine motifs on the cytoplasmic tails of some of the signalling lymphocyte activation molecule (SLAM) family (SLAM1, SLAM3–7). Activated SAP is known to relay information to other signalling components such as Fyn.
Mutations in the gene Src homology 2 (SH2) domain protein 1A (SH2D1A) causes X-linked lymphoproliferative syndrome (XLP).
T cell gene transcription
The signalling cassettes activated by the T cell receptor (TCR) produce various cytosolic factors (signalling components and activated transcription factors) that enter the nucleus to initiate a programme of early gene transcription (Module 9: Figure T cell signalling map). Some of the key early genes that are activated code for the interleukin-2 (IL-2) signalling pathway, which is a classical autocrine mechanism that is critical for completing the signalling events that end with the onset of DNA synthesis (Module 9: Figure G1 proliferative signalling). The promoter region of the IL-2 gene is thus an example of one of the early genes. It has an array of response elements that bind different transcription factors activated by the different signalling cassettes. Such promoter regions integrate information relayed to them by a number of different transcription factors:
As a result of these early transcriptional events, genes such as those for IL-2 and the IL-2 receptor initiate the autocrine loop that results in the activation of the interleukin-2 (IL-2) signalling pathway that functions later in G1 to send signals to the nucleus to interact with the cell cycle control elements to switch on DNA synthesis (Module 9: Figure T cell signalling map).
Interleukin-2 (IL-2) signalling pathway
Interleukin-2 (IL-2) is a cytokine that functions through non-enzyme-containing receptors (Module 1: Figure cytokines). IL-2 provides the link between the earlier events initiated by the T cell receptor (TCR) to the cell cycle components necessary to initiate DNA synthesis (Module 9: Figure T cell signalling map). The IL-2 receptor (IL-2R) has three subunits (α, β and γ), which have to come together to form a functional unit. The IL-2 signalling pathway transfers information to the cell cycle components using the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signalling pathway(Module 2: Figure JAK/STAT heterogeneity). In addition, it may also use the PtdIns 3-kinase and mitogen-activated protein kinase (MAPK) signalling pathways.
B cell activation
B cell activation depends on a number of receptor mechanisms. The primary process is B cell antigen receptor (BCR) activation, but this response is modulated by information coming from both B cell stimulatory co-receptors (CD19) and B cell inhibitory co-receptors (CD22 and FcγRIIB).
B cell antigen receptor (BCR) activation
The B cell antigen receptor (BCR) appears early during the differentiation of B cells (Module 8: Figure B cell differentiation). One of the earliest steps in differentiation, which occurs in the pro-B cell stage, is the expression of Igα (CD79a) and Igβ (CD79b) that are key components of the BCR. These two signalling components begin to transmit information to control differentiation and continue to be a major component of the BCR in mature B cells. The BCR is a hetero-oligomeric complex consisting of these two Igα and Igβ signalling proteins associated with the antigen-binding immunoglobulin IgM receptor, which consists of two heavy chains and two light chains (Module 9: Figure B cell activation). When this IgM component detects antigen, a non-receptor tyrosine kinase called Lyn brings about the tyrosine phosphorylation of their immunoreceptor tyrosine-based activation motifs (ITAMs) located on the cytoplasmic regions of Igα and Igβ. The transmembrane protein tyrosine phosphatase CD45 is responsible for activating Lyn to initiate the following cascade of signalling events that emanate from the activated BCR.
The phosphorylated ITAMs on Igα and Igβ recruit and activate the non-receptor spleen tyrosine kinase Syk, which then recruits adaptors and various signalling transducers to form a large macromolecular signalling complex. A key function of Syk is to phosphorylate the B cell linker protein (BLNK) at several sites to provide docking sites to recruit various key signalling components such as Bruton's tyrosine kinase (Btk) and phospholipase Cγ2 (PLCγ2). The Btk activates PLCγ2 by phosphorylating Tyr753 and Tyr759. The enzymatic activity of both Btk and PLCγ2 is stimulated by PtdIns3,4,5P3, the lipid messenger of the PtdIns 3-kinase signalling pathway. The PtdIns3,4,5P3 stimulates Btk to phosphorylate PLCγ2 and the latter is also directly activated by this highly charged lipid. The increase in PtdIns3,4,5P3 formation is described in more detail in the section on the B cell stimulatory co-receptors.
Once PLCγ2 is activated, it hydrolyses PtdIns4,5P2 to release diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (InsP3). The DAG activates protein kinase Cβ (PKCβ) to contribute to the onset of proliferation. The InsP3 plays a primary role in activating proliferation by increasing the intracellular level of Ca2+ (Module 9: Figure B cell activation). The InsP3 binds to the InsP3 receptor to release Ca2+ from the endoplasmic reticulum (ER) and once this internal store is depleted the store-operated channels (SOCs) are activated to enhance the entry of external Ca2+. The mechanism of store-operated channels (SOCs) activation depends on an interaction between STIM proteins in the ER-sensing store depletion to send information to open the Orai channels in the plasma membrane. One of the actions of Ca2+ is to activate NFAT that leads on to the increase in proliferation.
The phosphorylated BLNK adaptor is also connected to other signalling pathways such as the MAP kinase signalling pathway and the Rac signalling mechanism (Module 9: Figure B cell activation). The adaptor growth factor receptor-bound protein 2 (Grb2) binds to one of the phosphorylated tyrosine residues and then assembles the signalling components of the MAP kinase signalling pathway that results in the activation of ERK1/2 that contributes to the onset of proliferation (Module 2: Figure ERK signalling). Another output signal from the BLNK signalling complex is the Rac signalling mechanism (Module 2: Figure Rac signalling), which is initiated by the guanine nucleotide-exchange factor Vav that then stimulates Rac to promote the membrane localization and activation of PtdIns4P 5-kinase (PIP5K in Module 9: Figure B cell activation). Following activation of PIP5K, the resulting local formation of PtdIns4,5P2 provides the substrate used by both PLCγ2 and the PtdIns 3-kinase. The latter is a key signalling component of the B cell stimulatory co-receptors.
The BCR is of interest because it appears early during B cell differentiation in the bone marrow (Module 8: Figure B cell differentiation) and plays a critical role in providing the proliferative and survival signals to drive B cells through their programme of differentiation. It is then retained in mature B cells to enable them to function as memory cells to respond to foreign antigens.
B cell linker protein (BLNK)
The B cell linker protein (BLNK), which is also known as Src homology 2 (SH2) domain-containing leukocyte protein of 65 kDa (SLP-65), is phosphorylated during B cell antigen receptor (BCR) activation to provide binding sites for both Bruton's tyrosine kinase (Btk) and phospholipase Cγ2 (PLCγ2) (Module 9: Figure B cell activation).
B cell stimulatory co-receptors
The B cell stimulatory co-receptors can fine tune the B cell antigen receptor (BCR) response by activating PtdIns 3-kinase signalling to provide the PtdIns3,4,5P3 to enhance the activity of both Bruton's tyrosine kinase (Btk) and phospholipase Cγ2 (PLCγ2) (Module 9: Figure B cell activation). The trimolecular complex consisting of CD19, CD21 and CD81 is recruited into the vicinity of the BCR complex when CD21 binds to the C3d complement component. CD19, which is a B cell-specific surface protein, first appears in the pre-B cell stage during B cell differentiation in the bone marrow (Module 8: Figure B cell differentiation). The non-receptor tyrosine kinase Lyn, which initiated the BCR activation process, is also responsible for phosphorylating the cytoplasmic domain of CD19 to provide a binding site for the Class 1A PtdIns 3-kinase. The regulatory subunit p85 attaches the catalytic p110 subunits to the phosphorylated tyrosine residue on CD19, thus positioning the enzyme close to the membrane where it can begin to generate the lipid messenger PtdIns3,4,5P3 (Module 9: Figure B cell activation).
B cell inhibitory co-receptors
The B cell inhibitory co-receptors regulate the amplitude of the B cell antigen receptor (BCR) response through a number of inhibitory mechanisms. One such inhibitory process depends on CD22, which is a type I membrane protein that first appears in the pre-B cell stage during B cell differentiation in the bone marrow (Module 8: Figure B cell differentiation). CD22 has seven immunoglobulin (Ig)-like extracellular domains and it is the V-set N-terminal Ig-like domain that binds to its ligand, which is the α2,6-linked sialic acid (2,6Sia) ligand found on the surface of many membrane proteins such as the IgM of the BCR or CD45. When it is cross-linked to such proteins, the immunoreceptor tyrosine-based inhibition motifs (ITIMs) on its cytoplasmic tail are phosphorylated to provide docking sites for both Src homology 2 (SH2) domain-containing inositol phosphatase 1 (SHIP1) and Src homology 2 (SH2) domain-containing protein tyrosine phosphatase 1 (SHP1) (Module 9: Figure B cell activation). SHIP-1 hydrolyses Ins1,3,4,5P4 and PtdIns3,4,5P3 and thus reduces the stimulatory effect of this lipid on both Bruton's tyrosine kinase (Btk) and phospholipase Cγ2 (PLCγ2). The primary function of SHP-1, which is a tyrosine phosphatase, is to inhibit the BCR signalling pathways by dephosphorylating the phosphotyrosine residues on components such as Syk, BLNK and CD19.
Another inhibitory co-receptor FcγRIIB, which also has ITIMs capable of recruiting both SHIP-1 and SHP1, carry out lipid and protein dephosphorylation reactions respectively, similar to those described for CD22.
Smooth muscle cell proliferation
The proliferation of smooth muscle cells plays an important role in wound healing, but this also occurs during vascular remodelling, which can have serious pathophysiological consequences when it leads to pulmonary vasoconstriction and hypertension.
A central role of Ca2+ in the control of cell proliferation (Module 9: Figure growth factor signalling) is nicely exemplified in the case of smooth muscle cell proliferation. A smooth muscle cell provides a good example of a proliferation/differentiation switch whereby a fully differentiated cell, which is carrying out its function of contraction, returns to the cell cycle and begins to proliferate. What is so interesting about this dual role of contraction and proliferation is that Ca2+ plays a role in controlling both processes. Proliferation is also regulated through growth factors acting through transcription factors such as ETS-like transcription factor-1 (Elk-1) and Krüppel-like factor 4 (KLF4) (Module 8: Figure smooth muscle cell differentiation). The microRNAs miR-143 and miR-145 play an important role in converting such proliferating cells back into differentiated smooth muscle cells.
This dual action of Ca2+ in smooth muscle cells is similar to what occurs in cardiac cells, where Ca2+ controls both contraction and the transcriptional events responsible for cardiac hypertrophy (Module 12: Figure hypertrophy signalling mechanisms). As for cardiac hypertrophy, it is reasonable to argue that normal levels of Ca2+ signalling regulate contraction, but when this becomes excessive, cell proliferation is induced. As for other examples of cell proliferation, it is likely that a prolonged period of Ca2+ signalling might be necessary to stimulate smooth muscle cell proliferation (Module 9: Figure SMC proliferation). In the case of pulmonary artery smooth muscle cells, this prolonged signalling seems to depend upon a constant influx of Ca2+ through canonical transient receptor potential 1 (TRPC1), i.e. one of the store-operated channels (SOCs) that carry out capacitative Ca2+ entry, similarly to the ICRAC used for lymphocyte activation (Module 9: Figure T cell Ca2+ signalling).
A process of angiogenesis carries out the growth and repair of blood vessels, during which pre-existing blood vessels give rise to new vessels (Module 9: Figure angiogenesis). Differentiated endothelial cells that line the inner surface of blood vessels are normally in a quiescent state (G0) in that they have a very low turnover, of the order of hundreds of days. During the process of angiogenesis, however, they return to the cell cycle in response to various growth factors. Angiogenesis is usually driven by local tissue demands for oxygen and glucose, or by various mechanical stresses. An important component of this angiogenic response is the activation of hypoxia-inducible factor (HIF), which is a transcription factor that increases the expression of a large number of components such as vascular endothelial growth factor (VEGF) that function to regulate cell proliferation (Module 4: Figure HIF functions). One of the main angiogenic mechanisms is the sprouting and branching of pre-existing vessels, a process triggered by VEGF, which acts on endothelial cells to induce them to proliferate and to migrate to form the new vessels as shown in the following sequence of events (Module 9: Figure angiogenesis):
The sequence of events that occur during angiogenesis are orchestrated by a number of cell–cell signalling mechanisms (Module 9: Figure angiogenesis signalling):
Alterations in angiogenesis have a number of severe pathological consequences, such as diabetic retinopathy and age-related macular degeneration. Tumour angiogenesis plays a vital role in tumour growth and metastasis.
Vascular endothelial growth factor (VEGF)
Vascular endothelial growth factor (VEGF) is one of the main growth factors responsible for regulating both angiogenesis (Module 9: Figure angiogenesis) and the endothelial regulation of paracellular permeability. VEGF is also released during wounding and inflammation (Module 11: Figure inflammation).
The VEGF family, which are glycoproteins secreted in the vicinity of blood vessels to induce angiogenesis, has five members: VEGF-A, VEGF-B, VEGF-C, VEGF-D and placental growth factor (PLGF).
Abnormal activation of choroidal neovascularization by VEGF may be responsible for age-related macular degeneration (AMD).
Vascular endothelial growth factor receptors (VEGFR)
There are three vascular endothelial growth factor receptor isoforms [VEGFR-1 (Flt-1), VEGFR-2 (Flk-1) and VEGFR-3 (Flt-4)] that play a role in regulating angiogenesis and vasculogenesis.
These VEGFRs are typical protein tyrosine kinase-linked receptors(Module 1: Figure stimuli for enzyme-linked receptors) that have slightly different functions. VEGFR-1, which responds to VEGF-A, VEGF-B and placental growth factor (PLGF), functions in monocyte migration and the organization of proper vessels. VEGFR-2, which is activated mainly by VEGF-A, plays a major role in stimulating the endothelial cells to proliferate, to migrate and to differentiate into new vessels during angiogenesis (Module 9: Figure angiogenesis). It is also responsible for stimulating the formation of nitric oxide (NO). VEGFR-3, which is activated by VEGF-C and VEGF-D, is mainly located on lymphatic vessels, where it functions to stimulate lymphangiogenesis. Physical interactions between endothelial cells and the extracellular matrix (ECM) are responsible for the mechanosensitive control of VEGF receptor expression.
These VEGFRs are capable of activating a number of signalling pathways (Module 9: Figure VEGF-induced proliferation):
The miR-126 plays a role in maintaining angiogenesis by reducing the expression of SPRED1, which inhibits the MAP kinase signalling pathway and the PtdIns 3-kinase regulatory p85β subunit that inhibits the PtdIns 3-kinase signalling pathway.
Mechanosensitive control of VEGF receptor expression
The physical interaction between endothelial cells and the underlying extracellular matrix (ECM) may be responsible for regulating transcription of the vascular endothelial growth factor receptor 2 (VEGFR2). This mechanical signal appears to be detected by p190RhoGAP, which normally acts to inhibit the Rho signalling mechanism by enhancing the hydrolysis of GTP (Module 2: Figure Rho signalling). Rho appears to influence the transcription of VEGFR2 by altering the balance between two competing transcription factors: TFII-I and GATA2
Astrocytes are induced to grow at the sites of brain injury.
Insulin-secreting β-cell proliferation
Fully differentiated /1insulin-secreting β-cells have been shown to proliferate in response to pancreatectomy in mice, which raises the possibility of devising strategies to replace damaged cells in diabetics. The survival and proliferation of β-cells is regulated by glucose and by various hormones such as /1glucagon-like peptide-1 (GLP-1), which appear to act synergistically to activate gene transcription by stimulating the transducers of regulated cyclic AMP response element-binding protein (CREB) (TORC) (/1Module 7: Figure β-cell signalling). β-Cell proliferation is also controlled by Ca2+ acting through the /1nuclear factor of activated T cells (NFAT) transcriptional cascade.
Parathyroid gland hyperplasia
Hyperplasia of the parathyroid gland occurs when the gland is being stimulated excessively due either to hypocalcaemia or to a decrease in the level of vitamin D. Under normal conditions, there is little proliferation of the chief cells, but this is rapidly reversed during intense stimulation as occurs during the fall in plasma Ca2+ that occurs during the secondary hyperparathyroidismbrought on by kidney disease.
The hyperplasia induced by a fall in level of vitamin D provides some clues as to the mechanisms responsible for controlling proliferation of the chief cells. One of the actions of the hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] is to promote the expression of cyclin-dependent kinase (CDK) inhibitors such as p21, which inhibit cell proliferation (Module 7: Figure PTH secretion). The onset of hyperplasia can be rapid and may be accelerated by expression of both the epidermal growth factor (EGF) receptor and its normal ligand TGFα.
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Published online 1 October 2014
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